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Rapid COVID-19 diagnosis in the hospital is essential, although this is complicated by 30%-50% of nose/throat swabs being negative by SARS-CoV-2 nucleic acid amplification testing (NAAT). Furthermore, the D614G spike mutant dominates the pandemic and it is unclear how serological tests designed to detect anti-spike antibodies perform against this variant. We assess the diagnostic accuracy of combined rapid antibody point of care (POC) and nucleic acid assays for suspected COVID-19 disease due to either wild-type or the D614G spike mutant SARS-CoV-2. The overall detection rate for COVID-19 is 79.2% (95% CI 57.8-92.9) by rapid NAAT alone. The combined point of care antibody test and rapid NAAT is not affected by D614G and results in very high sensitivity for COVID-19 diagnosis with very high specificity.

Original publication




Journal article


Cell Rep Med

Publication Date





COVID-19, D614G, SARS-CoV-2, point of care testing, rapid diagnoses, Aged, Aged, 80 and over, Antibodies, Viral, COVID-19, COVID-19 Testing, Female, Humans, Immunoassay, Male, Middle Aged, Neutralization Tests, Nucleic Acid Amplification Techniques, Point-of-Care Testing, SARS-CoV-2, Sensitivity and Specificity, Spike Glycoprotein, Coronavirus