Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

The zinc finger transcription factor Gli3 is an essential mediator of hedgehog signaling. Gli3 has a dynamic expression pattern during embryonic development. In the neural tube, Gli3 transcripts are patterned along the anteroposterior and dorsoventral axes such that the initial broad expression in the posterior neural tube becomes dorsally restricted as neurogenesis takes place. Little is known about the molecular mechanisms that regulate this dynamic expression. Here, we report on a phylogenetic analysis of the Gli3 locus that uncovered a novel regulatory element, HCNE1. HCNE1 contains a compound Pbx/Meis binding site that binds Pbx and Meis/Prep proteins in vitro and in vivo. We show that HCNE1 recapitulates Gli3 expression in the developing neural tube and that mutations in the Pbx/Meis binding site affect the spatiotemporal control of HCNE1 transcriptional activity. Ectopic expression or loss of function of Pbx and Meis/Prep proteins in the chick and mouse embryo results in aberrant expression of endogenous Gli3 transcripts. We propose a novel role for TALE proteins in establishing the correct spatiotemporal expression pattern of Gli3 in the vertebrate spinal cord, thus implicating TALE transcription factors in early embryonic patterning events controlled by Sonic hedgehog signaling.

Original publication

DOI

10.1128/MCB.00451-10

Type

Journal article

Journal

Mol Cell Biol

Publication Date

04/2011

Volume

31

Pages

1432 - 1443

Keywords

Animals, Base Sequence, Binding Sites, Chickens, Embryo, Mammalian, Enhancer Elements, Genetic, Gene Expression Regulation, Developmental, Genetic Loci, Genome, Homeodomain Proteins, Humans, Introns, Kruppel-Like Transcription Factors, Mice, Models, Biological, Molecular Sequence Data, Nerve Tissue Proteins, Neural Tube, PC12 Cells, Protein Binding, Protein Multimerization, Rats, Time Factors, Transcription Factors, Transcription, Genetic