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A technique is described for preparing neutrophils (PMN) from small volumes of porcine blood as would be obtained from newborn piglets. The method involves dilution of anticoagulated blood to 50% with phosphate buffered saline followed by two steps of hypotonic lysis. The bactericidal efficiency of PMN produced in this way was shown to be unimpaired. © 1985.

Original publication

DOI

10.1016/0007-1935(85)90084-3

Type

Journal article

Journal

British Veterinary Journal

Publication Date

01/01/1985

Volume

141

Pages

355 - 361