Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Bacterial meningitis is a major global cause of morbidity and mortality. Rapid identification of the aetiological agent of meningitis is essential for clinical and public health management and disease prevention given the wide range of pathogens that cause the clinical syndrome and the availability of vaccines that protect against some, but not all, of these. Since microbiological culture is complex, slow, and often impacted by prior antimicrobial treatment of the patient, molecular diagnostic assays have been developed for bacterial detection. Distinguishing between meningitis caused by Neisseria meningitidis (meningococcus), Streptococcus pneumoniae (pneumococcus), Haemophilus influenzae, and Streptococcus agalactiae and identifying their polysaccharide capsules is especially important. Here, we review methods used in the identification of these bacteria, providing an up-to-date account of available assays, allowing clinicians and diagnostic laboratories to make informed decisions about which assays to use.

Original publication

DOI

10.1016/j.ebiom.2021.103274

Type

Journal article

Journal

EBioMedicine

Publication Date

03/2021

Volume

65

Keywords

Bacteria, LAMP assay, Meningitis, Molecular diagnostics, PCR, RT-PCR, Whole Genome Sequence data, DNA, Bacterial, Haemophilus influenzae, Humans, Latex Fixation Tests, Meningitis, Bacterial, Neisseria meningitidis, Nucleic Acid Amplification Techniques, Point-of-Care Systems, Streptococcus agalactiae, Streptococcus pneumoniae